《植物生理学报》 2014, 50(11): 1727-1732

松潘乌头块根愈伤组织诱导及再生体系的建立

雷颖*

甘肃林业职业技术学院园林工程系, 甘肃天水741020

通信作者：雷颖；E-mail: leiyinggl@163.com；Tel: 0938-2111090

摘　要：

以野生松潘乌头块根为材料, 进行愈伤组织诱导与植株再生培养。由于松潘乌头离体培养时, 组织褐变严重, 因此在愈伤组织诱导前须进行除褐培养, 培养基以MS+6-BA 1.0 mg•L^-1+VC 300 mg•L^-1+PVP 200 mg•L^-1效果较好, 连续转移3次后褐变率显著降低到10%。适宜的愈伤组织诱导培养基为MS+2,4-D 3.0 mg•L^-1+6-BA 2.0 mg•L^-1+LH 500 mg•L^-1, 诱导率100%; 不定芽分化培养基为MS+ZT 1.0 mg•L^-1+6-BA 2.0 mg•L^-1+NAA 0.5 mg•L^-1, 分化率93%; 不定芽增殖培养基为MS+6-BA 2.0 mg•L^-1+NAA 0.3 mg•L^-1, 平均增殖倍数为6.0左右; 生根培养基为1/2MS+IAA 0.3 mg•L^-1+VC 100 mg•L-¹ (或PVP 300 mg•L^-1), 平均生根数10条左右, 生根率为96%以上。将瓶苗移栽于森林土和蛭石以1:1 (V/V)混合的基质中, 生长良好, 成活率达90%以上。

关键词：松潘乌头; 块根; 愈伤组织诱导; 植株再生

收稿：2014-10-08   修定：2014-10-27

资助：兰州市科技计划项目(2011-1-140)。

Callus Induction and Establishment of Regeneration System of Aconitum sungpanense Hand.-Mazz. Root

LEI Ying*

Department of Landscape Engineering, Gansu Forestry Technological College, Tianshui, Gansu 741020, China

Corresponding author: LEI Ying; E-mail: leiyinggl@163.com; Tel: 0938-2111090

Abstract:

Aconitum sungpanense root was taken as the experimental material to make a study of callus induction and plant regeneration. The results showed that during in vitro culture of A. sungpanense, the tissues suffered from severe browning, and soit was indispensible to make the control of browning before callus induction. The medium of MS+6-BA 1.0 mg•L^-1+VC 300 mg•L^-1+PVP 200 mg•L^-1 was beneficial to browning controlling, which was shown by the fact that the browning rate significantly reduced to 10% after three continuous transfer. The medium of MS+2,4-D 3.0 mg•L^-1+6-BA 2.0 mg•L^-1+LH 500 mg•L^-1 was the best choice for callus induction and the induction rate rose by 100%. The medium of MS+ZT 1.0 mg•L^-1+6-BA 2.0 mg•L^-1+NAA 0.5 mg•L^-1 was suitable for adventitious shoot differentiation, with a rate of 93%. The medium of MS+6-BA 2.0 mg•L^-1+ NAA 0.3 mg•L^-1 was optimum for adventitious shoot proliferation and the average proliferation rate amounted to 6.0. The medium of 1/2MS+IAA 0.3 mg•L^-1+VC 100 mg•L^-1 (or PVP 300 mg•L^-1) was effective in rooting, which was indicated by the number of roots per shoot (about 10) and by the higher rooting rate (over 96%). The plantlets were transplanted to the growth matrix composed of forest soil and vermiculite (1:1, V/V) and grew perfectly, with a survival rate of 90%.

Key words: Aconitum sungpanense; root; callus induction; plant regeneration